Meanwhile , there was different in the development of embryos reconstructed with various strain oocytes . the activation methods , passages and types of es cells were comparing in mouse cloning 在胚胎干细胞异种克隆中，比较了激活重构胚的不同方法、不同代数胚胎干细胞以及不同类型的供核细胞对异种重构胚的影响。
In order to know if the genetic constitution of recipient oocytes influenced the development of embryos reconstructed from es cell , oocytes of c57bl / 6j and kunming albino were used as recipient , respectively 为了了解受体卵的遗传内涵是否对胚胎干细胞重构胚发育也会产生影响，分别以c57bl 6j和昆明白小鼠的m期去核卵为受体，构建胚胎干细胞重构胚。
Human bone morphogenetic protein 3 is a member of tgf - b superfamily . lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell . and is important to bone self - repairment and bone development during embryo morphogenesis . in addition , some other biological activities of hbmp - 3 have also been found . such as inducing development of embryo and stimulating differentiation of neural and blood cells . therefore , there is a great prospect in the use of hbmp - 3 . there is trace content of hbmp - 3 in human body . it has been expressed in the expression system of eukaryotes and prokaryotes respectively , but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system 人骨形成蛋白3 ( hbmp - 3 )属于tgf -超家族的一员，可以诱导间充质细胞分化为软骨和骨，在胚胎时期骨骼发育和骨再生修复中起着重要的作用，而且对胚胎发育过程中中胚层的诱导和分化、造血组织的发育以及神经系统的发育和修复等都起着重要作用，因而hbmp - 3有广阔的市场前景。它在人体内含量极微，尽管研究人员已经在原核细胞和真核细胞表达系统中分别进行了表达，但是由于原核表达系统缺乏翻译后的加工修饰，真核表达系统存在成本高、产量低等特点，限制了其在临床上的应用。
